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. 2015 Dec 18;291(8):4107–4120. doi: 10.1074/jbc.M115.681361

FIGURE 4.

FIGURE 4.

Icsbp target genes were differentially regulated in Icsbp−/− mice versus wild-type mice during emergency granulopoiesis. A, serum levels of IL1β and G-CSF were not significantly different in WT versus Icsbp−/− mice during steady-state or emergency granulopoiesis. Mice were injected with Alum to induce emergency granulopoiesis or saline control and sacrificed after 2 weeks. Serum IL1β and G-CSF levels were determined by ELISA. Statistically significant differences are indicated by *, **, ***, and #. B, expression of phagocyte effector Icsbp target-genes increased in the bone marrow of WT mice but not Icsbp−/− mice during emergency granulopoiesis. Bone marrow from the mice described above was analyzed for gene expression by real-time PCR. Statistically significant differences are indicated by *, **, ***, #, ##, and ###. C, expression of Icsbp target genes that influence proliferation/survival (Fap1 and Gas2), β-catenin target genes (c-myc and survivin), and genes required for emergency granulopoiesis (Stat3 and C/EBPβ) increased initially during emergency granulopoiesis in WT mice and returned to baseline at 4 weeks, but increased expression was sustained in Icsbp−/− mice. Mice were sacrificed before or 2–4 weeks after Alum injection, and gene expression in CD34+ bone marrow cells was determined. Statistically significant differences are indicated by *, **, ***, #, ##, and ##. p < 0.02 was considered statistically significant.