Skip to main content
. 2015 Dec 18;291(8):4107–4120. doi: 10.1074/jbc.M115.681361

FIGURE 7.

FIGURE 7.

Expression of Fanconi C and F was impaired in the bone marrow of Icsbp−/− mice during emergency granulopoiesis, and susceptibility to DNA damage was increased. A, expression of the Icsbp target genes FANCC and FANCF in the bone marrow during emergency granulopoiesis was decreased in Icsbp−/− mice versus WT mice. Icsbp−/− and WT mice were injected with Alum to induce emergency granulopoiesis or saline control and sacrificed 2 weeks later. Bone marrow was analyzed by real-time PCR for expression of Fanconi C or F mRNA. Statistically significant differences are indicated by *, **, ***, and #. B, Icsbp−/− bone marrow exhibited increased DNA breaks and radials in comparison with WT bone marrow after G-CSF stimulation. CD34+ bone marrow cells from Icsbp−/− or WT mice were treated with G-CSF and analyzed for mitomycin C-induced DNA breaks or radials in chromatin spreads. Statistically significant differences are indicated by * and **. p < 0.02 was considered statistically significant.