FIGURE 5.

Cold stimulation enhanced the expression of UCP1 mRNA and protein in Prip-KO BAT. RD-fed 10-week-old wild-type (WT) and Prip-KO (KO) mice kept at 4 °C for 6 h (A–D) or 30 °C for 24 h (C and D) were used for the experiments. A, Ucp1, Pgc1α, Dio2, Pparα, Pparδ, and Pparγ mRNA expression in BAT was analyzed by qRT-PCR (WT and KO, n = 3). The WT value is set to 1. B, UCP1 protein expression in BAT was analyzed by immunoblotting (WT and KO, n = 3). β-Actin was used as the loading control. Bands were quantified using ImageJ software. Mean values normalized to β-actin are shown. The data represent the mean ± S.E. *, p < 0.05; **, p < 0.01 versus the corresponding WT value. C, phosphorylation of HSL (p-HSL S660) in BAT at 30 and 4 °C. A set of representative images from 3 independent experiments is shown. HSL was used as the loading control. D, Ucp3 mRNA expression in the skeletal muscle at 30 and 4 °C was analyzed by qRT-PCR (WT and KO, n = 3). Data are expressed as the mean ± S.E. of relative gene expression.