FIGURE 1.

Synthesis of NA-17. A, structures of the eight naphthalimides compounds. B, synthetic route to NA-17. Reagents and conditions are as follows: a, 3,4-methylenedioxyphenethylamin, EtOH, 70 °C, 10 h; b, 3-dimethylaminopropylamine, DMSO, 135 °C. C, mass spectrum of NA-17. D, the purity of NA-17 determined by HPLC. The column used was a reversed-phase C₁₈ column (YMC American Inc. HPLC column, 150 mm × 4.6-mm inner diameter). The column temperature was 35 °C. The mobile phase was methanol/H₂O (from 10:90 to 100:0) with 0.01% TFA with a flow rate of 1.0 ml/min. The injection volume was 20 μl. mAU, milliabsorbance units.