FIGURE 2.

FLNa depletion stabilizes tetherin expression. A, HA-tetherin-expressing A7 (left panel) and M2 (right panel) cells were treated with cycloheximide for the indicated times (0, 2, 4, and 6 h), followed by Western blotting analysis with antibodies against HA and actin. B, A7 (left panel) and M2 (right panel) cells were treated with cycloheximide for the indicated times (0, 1, 2, and 4 h), followed by immunoblot analysis using anti-tetherin and anti-actin antibodies. C, densitometry values of tetherin (about 30–36 kDa) signal intensities at each time point normalized to the densitometry value at the first time point, which was set to 100%. The results shown represent the mean ± S.D. (n = 3).