TABLE 1.
Sequences of primers used for mutagenesis and qPCR
| Usage | Gene | Forward primer | Reverse primer |
|---|---|---|---|
| Mutagenesis | Tet2 H1302Y | GCTCATTCCTACAGAGACCAGCAGAACATGC | GCATGTTCTGCTGGTCTCTGTAGGAATGAGC |
| Mutagenesis | Tet2 D1304A | GCTCAATTCCCACAGAGCCCAGCAGAACATGC | GCATGTTCTGCTGGGCTCTGTGGGAATGAGC |
| Mutagenesis | Tet2 H1802R | GCAAAGTGTGAGGTTCGTGCCACAACC | GGTTGTGGCACGAACCTCACACTTTGC |
| Mutagenesis | Tet2 R1817M | CCCCACCATGATCTCACTTGTACTGTATAGG | CCTATACAGTACAAGTGAGATCATGGTGGGG |
| Mutagenesis | Tet2 R1817S | CCCCACCAGTATCTCACTTGTACTGTATAGG | CCTATACAGTACAAGTGAGATACTGGTGGGG |
| qPCR | TBP | GAATATAATCCCAAGCGGTTT | ACTTCACATCACAGCTCCCC |
| qPCR | TET1 | GAGAATAGGTATGGTCAAAA | CTTCATCACTGCTTCTTCTT |
| qPCR | TET2 | TGCCGTCTGGGTCTGAAG | CCTCAGGTTTTCCTCCAAAT |
| qPCR | TET3 | CGTCGAACAAATAGTGGAGA | CTTTCCCCTTCTCTCCATAC |
| qPCR | VEGFA | AGGAGGAGGGCAGAATCATCA | ATGTCCACCAGGGTCTCGATTG |
| qPCR | BNIP3 | CCACCTCGCTCGCAGACACCAC | GAGAGCAGCAGAGATGGAAGGAAAAC |
| qPCR | PGK1 | CTGTGGGGGTATTTGAATGG | CTTCCAGGAGCTCCAAACTG |
| qPCR | HK2 | ATTGTCCAGTGCATCGCGGA | AGGTCAAACTCCTCTCGCCG |
| qPCR | ENO1 | TGGTGTCTATCGAAGATCCCTT | CCTTGGCGATCCTCTTTGG |
| qPCR | IDH1 | TGTGGTAGAGATGCAAGGAGA | TTGGTGACTTGGTCGTTGGTG |