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HPLC chromatogram of (a) standard tZ (1.0 mg mL−1, 0.05 N KOH), (b) overlap of adenine (near 3.0 min) with the 2.5 min degradant peak from tZ incubated at 85°C for 48 days, (c) adenine retention time shifting after exposure to 0.05 N HCl for 4 min relative to base exposure (0.05 N KOH) over the same time period.
