Figure 5.

Flotillin-1 activates the NF-κB and Wnt/β-catenin pathways in cervical cancer cells. A. Luciferase reporter activities analyzed in cells according to the TOP-flash versus FOP-flash intensity scale. B. Real-time PCR indicates an apparent overlap between Wnt/β-catenin-dependent gene expression and flotillin-1-regulated gene expression. Pseudo-color represents the intensity scale of flotillin-1 shRNA versus RNAi vector or flotillin-1 cDNA vs. Vector, generated by log₂ transformation. C. NF-κB luciferase reporter activity. D. Real-time PCR indicates an apparent overlap between NF-κB-dependent gene expression and flotillin-1-regulated gene expression. Pseudo-color represents the intensity scale of flotillin-1 cDNA vs. Vector or flotillin-1 shRNA versus RNAi-vector, generated by log₂ transformation. Vector: cells transfected with vector; OV: cells transfected with flotillin-1 cDNA; RNAi-vector/Scramble: cells transfected with scramble shRNA; RNAi#1/RNAi#2/Ri: cells transfected with two human shRNA sequences to repress flotillin-1.