Table 2.
Selected primers for gene amplification and sequencing
| Primers | Sequence | Annealing Temperature | Base pair |
|---|---|---|---|
| EGFR F exon 2 | CCAAGGCACGAGTAACAAGCT | 56°C | 132 |
| EGFR R exon 2 | TCATAATTCCTCTGCACATAGG | ||
| EGFR F exon 3 | TAGACCTTGAGTTCTTGAGTTC | 56°C | 155 |
| EGFR R exon 3 | CATATTTCCTCTGATGATCTGC | ||
| EGFR F exon 4 | AGTGCTCACCGCAGTTCCAT | 55°C | 228 |
| EGFR R exon 4 | CAGTGCTGTAGAGCTGTCC | ||
| EGFR F exon 5 | TATTGAATGTGCTTAACTCAGG | 58°C | 189 |
| EGFR R exon 5 | CATGGGTCTGAGGCTGTTC | ||
| EGFR F exon 6 | CTTCAGCTCACAGGGAACCTT | 57°C | 236 |
| EGFR R exon 6 | CACAGGAAGTCTTCTGTCCTG | ||
| EGFR F exon 7 | GAGTGTACTTACCTCACTTGC | 58°C | 168 |
| EGFR R exon 7 | GTGGCACCAAAGCTGTATTTG | ||
| EGFR F exon 18 | GCTTGCAAGGACTCTGGGCT | 62°C | 360 |
| EGFR R exon 18 | CCAAACACTCAGTGAAACAAAGAG | ||
| EGFR F exon 19 | GTGCATCGCTGGTAACATCCA | 55°C | 306 |
| EGFR R exon 19 | CATTTAGGATGTGGAGATGAGC | ||
| EGFR F exon 20 | GAAACTCAAGATCGCATTCATGC | 60°C | 379 |
| EGFR R exon 20 | GCAAACTCTTGCTATCCCAGGAG | ||
| EGFR F exon 21 | CTAACGTTCGCCAGCCATAAGTCC | 57°C | 370 |
| EGFR R exon 21 | GCTCACCCAGAATGTCTGGA | ||
| EGFR F exon 22 | CACTCGTAATTAGGTCCAGAG | 55°C | 295 |
| EGFR R exon 22 | CTCAGTACAATAGATAGACAGCAATG | ||
| EGFR F exon 23 | CAAGACTACAGAAATGTAGGTTTC | 63°C | 373 |
| EGFR R exon 23 | GTGATGACATTTCTCCAGGGATGC | ||
| EGFR F exon 24 | CATCACCAATGCCTTCTTTAAGC | 59°C | 310 |
| EGFR R exon 24 | GCTGGAGGGTTTAATAATGCGATC |