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Expression of p53 and downstream effectors in U2OS and MG-63 cells after doxorubicin treatment. A. Expression of wild-type p53 was examined in MG-63, U2OS and HEK-293T cells by Western blot. B, C. U2OS and MG 63 cells were incubated with 2 µg/mL doxorubicin for 0, 12, 24 or 48 hours. Levels of PUMA, p21 and cleaved Caspase 3 were determined by Western blot. GAPDH was used as a loading control.
