Skip to main content
. 2016 Jan 15;8(1):1–11.

Figure 4.

Figure 4

IRS1 is a direct target of miR-144a. A. The predicted binding sites for miR-144 in the 3’UTR of IRS1 and the mutations in the binding sites are shown. B. The luciferase activity of the wild type IRS1 3-UTR (Wt) and mutant IRS1 3’UTR (Mut) co-transfected with miR-144 or miR-NC was determined. C. IRS1 expression on mRNA level in Hep2 cells transfected with miR-144 mimic or miR-NC were determined by qRT-PCR. D. IRS1, AKT and p-AKT protein expression were determined in Hep2 cells transfected with miR-144 mimic or miR-NC by western blot. qRT-PCR and western blot data were normalized to β-actin. *P < 0.05, **P < 0.01 versus miR-NC. E. qRT-PCR determined IRS1 mRNA expression in 24 cases of LSCC tissues and matched normal tissues. The β-actin was used as an internal control. *P < 0.05, **P < 0.01 versus normal tissues. F. The reverse relationship between IRS1 mRNA expression and miR-144 expression was determined by Pearson’s correlation.