Figure 1.

Detection of HOTAIR expression in LoVo CD133⁺CSCs and single-CD133⁺-ShHOTAIR clone isolation. A. HOTAIR expression in CD133⁺CSCs and wild type of LoVo cells identified by qRT-PCR. B. HOTAIR expression in CD133⁺CSCs-ShHOTAIR and CD133⁺CSCs-Scramble cells identified by qRT-PCR. C. A single-CD133⁺-ShHOTAIR clone isolated from the ShHOTAIR transfected CD133⁺ clones with 800 μg/ml G418 on Day 2, Day 4, Day 7, and Day 14, respectively, viewed under a light microscope (top) or a fluorescence microscope (bottom). D. The selected CD133⁺-ShHOTAIR or CD133⁺-Scramble clones were viewed under a light microscope (top) or fluorescence microscope (bottom). The p values were from the Student’s t test and indicated if the differences were statistically significant (p < 0.05).