Figure 4.

CD133⁺-ShHOTAIR reduce its tumorigenicity and metastatic potential in mice. A. Images represent the tumor growth in the nude mice 30 days after they were injected with 5×10⁵ LoVo CD133⁺WT or CD133⁺-Scramble or CD133⁺-shHOTAIR. B. Images of tumor tissue sizes and quantity in 30 days. C, D. Tumor volume and the tumor-free mice injected with the CD133⁺WT or CD133⁺-Scramble or CD133⁺-shHOTAIR. E. Images of lung tissues taken from the mice 30 days after they were injected with the CD133⁺WT or CD133⁺-Scramble, and lung tissue images from the mice 45 days after they were injected with the CD133⁺-shHOTAIR. Presence of metastatic tumor nodes in lungs is visible in the mice injected with the CD133⁺WT or the CD133⁺-Scramble. F. Quantification of the lung metastatic tumor nodes, referring to the differences as indicated, which was analyzed by using the Student’s t test method.