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CTGF promotes transition of HSCs into myofibroblast-like cells in vitro. (A) The CTGF-modified HSCs were then put in culture to allow automatic transition into myofibroblast-like cells. The phenotypic changes of the cells were monitored at 3 day (early stage), 5 day (middle stage) and 7 day (activated stage). (B, C) The levels of α-SMA in control HSCs (SCR) andCTGF-overexpressing HSCs (CTGF), by RT-qPCR (B), and by Western blot (C). *p<0.05. NS: non-significant. N=5.
