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5azadCyd reduces CTGF gene promoter methylation in vitro. A. We used 5aza2’deoxycytidine (5azadCyd) to treat cultured CTGF-modified HSCs and analyzed the phenotypic changes of the cells at 3 day (early stage), 5 day (middle stage) and 7 day (activated stage). B. MSP analysis of the CTGF gene promoter methylation in HSCs. C. The quantification of CTGF gene promoter methylation by BS. *p<0.05. NS: non-significant. N=5.
