Figure 4.

miR‐222 blocks cisplatin‐induced cell death through activation of the Akt pathway. (A–D) T24 (A and B) and 5637 (C and D) cells were treated as indicated and Western blotting was performed to detect the phosphorylation status of Akt (A and C) and the p‐Akt/Akt ratio (B and D). (E and F) T24 (E) and 5637 (F) cells were treated with LY294002 (20 μM) and/or CDDP (2.5 mg/l) as indicated, and cell viability was measured with Cell Counting Kit‐8 assay. (G–I) T24 (G and H) and 5637 (G and I) cells were treated as indicated, and the cleavage of caspase‐3 was detected by western blotting. Data are expressed as the mean ± S.D. of at least three independent experiments. **P < 0.01, between the indicated groups.