Skip to main content
. 2016 Jan 22;20(3):559–567. doi: 10.1111/jcmm.12760

Figure 5.

Figure 5

miR‐222 activates the mTOR pathway and inhibits autophagy in bladder cancer cells. (A and B) T24 (A) and 5637 (B) cells were transfected with the miR‐222 mimic and the phosphorylation of p70S6K 24 hrs after transfection was detected by using Western blotting. (C and D) T24 (C) and 5637 (D) cells were transfected as described above for 24 hrs. Rapamycin (RP, 20 nM) and/or CDDP (2.5 mg/l) were added as indicated, and the cells were incubated for 24 hrs. Cell viability were detected by using the Cell Counting Kit‐8 assay. (E and F) T24 cells were treated as described in (A and B), and LC3 lipidation was determined by Western blotting. (G and H) T24 cells were transfected with the EGFP‐LC3 plasmid and treated as indicated. The number of EGFP‐LC3 puncta was counted by using confocal microscopy. Data are expressed as the mean ± S.D. of at least three independent experiments. **P < 0.01, versus the indicated groups.