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. 2015 Dec 21;20(3):441–449. doi: 10.1111/jcmm.12738

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© 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Immunoprecipitation analysis and mRNA levels in PBMCs. (A) Card9mRNA expression; (B) Bcl10 mRNA expression; (C) immunoprecipitation of Card9‐Bcl10 complex (input: 1, 4, 7, 10, 13, 16; anti‐Bcl‐10 immunoprecipitated complex: 2, 5, 8; anti‐Card9 immunoprecipitated complex: 11, 14, 17; lank control: 3, 6, 9, 12, 15, 18). Immunoprecipitation analysis was representative from 20 healthy volunteers, 35 MAP and 17 SAP patients. PCR results were means ± S.D. of measurement on day 1, 3 and 5 (n_{controlled group} = 20, n_{MAP patients} = 17, n_{SAP patients} = 35). a: control versus SAP. MAP, P < 0.05; b: SAP on day 1 versus MAP on day 1, P < 0.05; c: SAP on day 3 versus MAP on day 3, P < 0.05.