Skip to main content
. 2016 Jan 12;20(3):495–505. doi: 10.1111/jcmm.12754

Figure 4.

Figure 4

Experimental establishment of Apelin‐13 as a target of microRNA miR‐503. (A) Sequences of miR‐503 binding sites in Apelin‐13. (B) The luciferase reporter assay results depicting the activities of chimeric vectors carrying the luciferase gene and a fragment of the Apelin‐13 3′‐untranslated region from rats containing the binding sites of miR‐503. (C) is mRNA and (D) is the protein level of Apelin‐13 induced by overexpression of miR‐503 in cultured neonatal mice cardiac fibroblasts (CFs). Data are expressed as the means ± S.E.M.; n = 4. NC indicates negative control. #P < 0.05 versus NC; *P < 0.05 versus miR‐503.