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. 2016 Jan 12;20(3):506–517. doi: 10.1111/jcmm.12756

Figure 5.

Figure 5

PGRN deficiency increased the production of systemic and local pro‐inflammatory mediators after injection of LPS. ELISA of serum levels of inflammatory mediators including tumour necrosis factor α (TNF‐α) (A), interleukin 6 (IL‐6) (B), IL‐10 (C) and monocyte chemoattractant protein 1 (MCP‐1) (D). (E) Western blot analysis of cold‐inducible RNA‐binding protein (CIRP) and high mobility group box protein 1 (HMGB1) in the serum from WT and Grn −/− mice with LPS injection at 6 and 24 hrs. PS red, Ponceau S red staining. Real‐time RT‐PCR analysis of mRNA levels of TNF‐α (F), IL‐6 (G), IL‐1β (H) and IL‐10 (I) in the lung after LPS injection. Data are mean ± S.D. *P < 0.05; **P < 0.01 compared with WT mice (n = 6 mice/group).