Table 1. Differential expression of the selected genes in roots of cassava cultivar F01.
| GenBank accession no. of gene | Description | P value inT test | Fold change in expression in F01 roots |
|---|---|---|---|
| F01 vs. H124 at ‘seedling’ | |||
| Antiporter/Transporter | |||
| FG805259 | Glucose transporter type 12 | 0.003 | 1.5 |
| FG804711 | ATP:ADP antiporter | 0.004 | 1.7 |
| Starch metabolism related enzyme | |||
| DQ017830 | Manihot esculenta alpha-amylase | 0.005 | 2.3 |
| Signaling | |||
| FG806408 | AtTLP3 (TUBBY LIKE PROTEIN 3) | 0.000 | 390.2 |
| FG805296 | Pyruvate kinase, cytosolic isozyme | 0.002 | 1.8 |
| FG806368 | pyrophosphate-dependent phosphofructo-1-kinas | 0.000 | 2.1 |
| F01 vs. H124 at formation of root system | |||
| Transporter | |||
| FG804711 | ATP:ADP antiporter | 0.000 | −2.18 |
| FG805197 | Potassium/chloride transporters | 0.001 | −1.92 |
| FG805211 | Organic cation transporter | 0.000 | −1.41 |
| FG805259 | Glucose transporter type 12 | 0.000 | −2.10 |
| FG806078 | Glucose-6-phosphate/phosphate-translocator | 0.000 | −5.01 |
| FG805678 | Chloroplast glucose-6-phosphate/phosphate translocator | 0.004 | −1.49 |
| Starch metabolism-related enzyme | |||
| DQ011041 | M. esculenta alpha-amylase 2 (amy2) | 0.000 | −2.93 |
| X74160 | M. esculenta granule-bound starch synthase | 0.000 | −1.93 |
| X74160 | M. esculenta granule-bound starch synthase | 0.000 | −1.93 |
| Sucrose synthase | |||
| BM260279 | Sucrose synthase | 0.000 | 1.35 |
| AY818397 | M. esculenta sucrose synthase | 0.000 | −9.10 |
| Signaling | |||
| FG805239 | Hexokinase | 0.000 | −2.98 |
| FG806408 | AtTLP3 (Tubby like protein 3) | 0.008 | −1.76 |
| FG805296 | Pyruvate kinase, cytosolic isozyme | 0.000 | −1.90 |
| FG806368 | Pyrophosphate-dependent phosphofructo-1-kinas | 0.004 | −1.60 |
| Growth and development | |||
| FG806385 | Auxin response factor-like protein | 0.001 | −1.66 |
| FG806743 | Secretory carrier membrane protein (SCAMP) family protein | 0.000 | 2.58 |
| FG806552 | Auxin-responsive factor TIR1-like protein | 0.004 | −1.25 |
| FG807559 | Putative ripening-related protein | 0.002 | −1.25 |
| FG805555 | Late embryogenesis abundant protein 5 | 0.000 | −3.13 |
| FG807051 | Late embryogenesis abundant protein Lea5 | 0.000 | −3.81 |
| FG806890 | Induced stolon tip protein | 0.000 | −2.44 |
| FG806889 | Cell division ATP-binding protein FtsE | 0.005 | −1.84 |
| F01 vs. H124 at root bulking | |||
| Antiporter/Transporter | |||
| FG804711 | ATP:ADP antiporter | 0.008 | −1.22 |
| FG805321 | Inner-membrane translocator | 0.000 | 2.84 |
| FG805211 | Similar to Solute carrier family 22 (organic cation transporter) | 0.001 | 1.41 |
| Vascular system | |||
| FG806684 | GRP-like protein 2 | 0.000 | 1.37 |
| FG807243 | Fructose-bisphosphate aldolase, putative | 0.001 | 2.69 |
| Starch metabolism related enzyme | |||
| DQ017830 | M. esculenta alpha-amylase | 0.001 | 1.80 |
| X77012 | M. esculenta starch branching enzyme | 0.001 | 2.24 |
| X69714 | M. esculenta strach branching enzyme (r-3) | 0.001 | 1.20 |
| X74160 | M. esculenta granule-bound starch synthase | 0.000 | −4.51 |
| Sucrose synthase | |||
| BM260279 | Sucrose synthase | 0.002 | 1.35 |
| DQ443534 | M. esculenta sucrose synthase | 0.004 | 1.28 |
| AY818397 | M. esculenta sucrose synthase | 0.000 | −4.14 |
| Signaling | |||
| FG805239 | Hexokinase | 0.002 | 1.48 |
| FG805296 | Pyruvate kinase, cytosolic isozyme | 0.007 | 1.31 |
| FG806368 | Pyrophosphate-dependent phosphofructo-1-kinas | 0.000 | 4.27 |
| F01 vs. H124 at starch maturity | |||
| Antiporter/Transporter | |||
| FG806078 | Glucose-6-phosphate/phosphate-translocator | 0.000 | 1.69 |
| FG805211 | Similar to Solute carrier family 22 | 0.005 | 1.34 |
| FG805259 | Glucose transporter type 12 | 0.000 | 1.67 |
| Vascular system | |||
| FG806684 | GRP-like protein 2 | 0.003 | 1.37 |
| Starch metabolism related enzyme | |||
| DQ017830 | M. esculenta alpha-amylase | 0.000 | 3.21 |
| AY944584 | M. esculenta beta-amylase (AmyB) | 0.004 | 2.51 |
| X74160 | M. esculenta granule-bound starch synthase | 0.000 | −3.02 |
| EF667960 | M. esculenta starch synthase isoform I (SSI) | 0.006 | −1.37 |
| Sucrose synthase | |||
| AY818397 | M. esculenta sucrose synthase | 0.005 | −4.28 |
| DQ443534 | M. esculenta sucrose synthase | 0.005 | 1.40 |
| BM260279 | Sucrose synthase | 0.000 | 1.74 |
| DQ443534 | Sucrose synthase | 0.005 | 1.40 |
| Signaling | |||
| FG805239 | Hexokinase | 0.001 | 1.35 |
| FG805296 | Pyruvate kinase, cytosolic isozyme | 0.006 | 1.35 |
| Growth and development | |||
| FG805555 | Late embryogenesis abundant protein 5 | 0.000 | 2.00 |
| FG804941 | Growth-on protein GRO10 | 0.005 | 1.21 |
| FG807273 | Expansin-like protein precursor | 0.000 | 2.15 |
| FG807559 | Putative ripening-related protein | 0.001 | −1.45 |
| FG807051 | Late embryogenesis abundant protein Lea5 | 0.000 | −5.44 |
| DN740362.1 | Similar to ethylene response factor | 0.003 | 1.50 |
| FG807216 | Ethylene response factor | 0.004 | 1.22 |
The plants were propagated by stem stakes and grown in the experimental fields of Agricultural College of Guangxi University in the 2010–2011 growing season following conventional cultivation and management. The expression of each gene in F01 and H124 was analyzed by qRT-PCR with a pair of cDNA sequence-specific primers, respectively. The figures in column ‘Fold change’ indicate a differential expression levels of the target genes in F01 roots under a comparison as F01 roots vs. H124 roots at the same growth stage, which was calculated following the formula of 2−ΔΔCt(ΔCtF01 roots−ΔCtH124 roots. The bold italic figures and negative figures in column ‘Fold change’ indicate the up-regulated and down-regulated expression levels of the genes, respectively, The up-regulated and down-regulated expression was determined according to a cutoff fold of ≧1.2 or ≦−1.2 at p < 0.01, respectively.