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. 2016 Feb 19;6:21094. doi: 10.1038/srep21094

Figure 8. LPS and LTA in presence of morphine differentially modulates bacterial killing.

Figure 8

J774 cells undergoing morphine treatment (1 μM overnight), were treated with LPS (50 ng/ml) or LTA (10 μg/ml) for 4 hours (in antibiotic free media) prior to addition of GFP E. coli (A) and S. pneumoniae (B). Following 30 min of phagocytosis cells were washed four times with PBS containing 1% penicillin/streptomycin and then washed with antibiotic free medium and incubated overnight in antibiotic free media containing morphine, LPS or LTA (where appropriate). Next day cells were lysed and plated on LB plates (Ampicillin 100 μg/ml) for GFP E. coli and Kanamycin (50 ug/ml) blood agar plates for S. pneumoniae, and evaluated for bacterial colony formation (CFU) the next day. Significance was determined using student’s t-test (*p < 0.05, **p < 0.001,***p < 0.0001).