Figure 3. SQR9 enhances DC maturation.

DCs were incubated in B. amyloliquefaciens SQR9 (10⁶ CFU, 10⁷ CFU or 10⁸ CFU) or cholera toxin (CT, 2 μg/mL). After 24 h, DCs and supernatants were collected to detect DCs phenotypes (A–H) and DC-mediated cytokine secretion (I–L). The expression of CD40 (A,E), CD80 (B,F), CD86 (C,G) and MHC-II (D,H) by DCs was analyzed by FACS. The secretion of IL-10 (I), IL-12p70 (J), IL-6 (K) and TNF-α (L) in culture supernatants was measured by ELISA. The experiments were repeated for three independent experiments. The data are presented as the means ± SD. N = 3. Error bars mean standard deviation (SD). *P < 0.05; **P < 0.01.