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. 2016 Feb 19;6:21363. doi: 10.1038/srep21363

Figure 4. SQR9 recruits DCs and increases the number of H9N2 WIV-loaded DCs in CLNs.

Figure 4

(A–D) C57BL/6 mice were intranasally administered PBS or DyLight 633-labeled H9N2 WIV (10 μg HA) with or without B. amyloliquefaciens SQR9 (107 CFU) or cholera toxin (CT, 2 μg) for 90 min. Epithelial cells from the nasal mucosa and cervical lymph node (CLN) cells were isolated and analyzed by FACS. (A,B) FACS analysis of FITC-CD11c+ cells from nasal mucosal epithelial cells. (C,D) FACS analysis of DyLight 633 virus-loaded DCs based on gating for FITC-CD11c+ cells from CLNs. (E,F) C57BL/6 mice were intranasally administered PBS or H9N2 WIV (10 μg HA) with or without B. amyloliquefaciens SQR9 (107 CFU) or cholera toxin (CT, 2 μg) for 12 h. (E,F) Chemokine (C-C Motif) Ligand 20 (CCL20) expression in the nasal mucosa and in CLNs was detected by real-time quantitative PCR. The experiments were repeated for three independent experiments. The data are presented as the means ± SD. N = 3. Error bars mean standard deviation (SD). *P < 0.05; **P < 0.01.