Figure 1. Experimental workflow from sample preparation to western blot and lipidomics analyses.

Three mitochondria isolation methods, differential centrifugation (DC), ultracentrifugation (UC) and magnetic bead-assisted isolation (MACS) were systematically compared; see Methods section for further details. SN, supernatant; MTBE, methyl tert-butyl ether; CE, cholesteryl ester; CER, ceramide; CL, cardiolipin; DAG, diacylglycerol; FFA, free fatty acids; HexCER, hexosyl-ceramide; LPC, lyso-phosphatidylcholine; LPE, lyso-phosphatidylethanolamine; LPI, lyso-phosphatidylinositol; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PE-P, phosphatidylethanolamine plasmalogen; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserine; SM, sphingomyelin; TAG, triacylglycerol.