Table 1. Summed content of lipid classes quantified by LC-MS lipid profiling of mitochondria purified using different isolation methods (pmol/μg protein).
| Lipid(sub-)class | DC |
UC |
MACS |
UC vs. DC | UC vs. MACS | DC vs. MACS | |||
|---|---|---|---|---|---|---|---|---|---|
| Mean | SD | Mean | SD | Mean | SD | p-value | p-value | p-value | |
| CE | 0.012 | 0.001 | 0.019 | 0.007 | 0.033 | 0.017 | n.s. | n.s. | 0.0135 |
| CER | 2.838 | 0.404 | 2.447 | 0.326 | 2.703 | 0.662 | n.s. | n.s. | n.s. |
| CL | 8.623 | 1.779 | 34.433 | 10.814 | 5.217 | 1.522 | <0.0001 | <0.0001 | n.s. |
| DAG | 0.092 | 0.007 | 0.129 | 0.018 | 0.283 | 0.077 | n.s. | 0.0002 | <0.0001 |
| FFA | 59.601 | 10.134 | 60.664 | 11.180 | 87.242 | 28.114 | n.s. | n.s. | n.s. |
| HexCER | 1.013 | 0.076 | 0.516 | 0.113 | 0.719 | 0.151 | <0.0001 | 0.0282 | 0.0033 |
| LPC | 1.625 | 0.459 | 1.834 | 0.476 | 1.709 | 0.727 | n.s. | n.s. | n.s. |
| LPE | 0.728 | 0.163 | 0.838 | 0.251 | 0.699 | 0.249 | n.s. | n.s. | n.s. |
| LPI | 0.081 | 0.032 | 0.064 | 0.010 | 0.052 | 0.021 | n.s. | n.s. | n.s. |
| PC | 91.406 | 5.802 | 86.358 | 11.261 | 89.657 | 14.345 | n.s. | n.s. | n.s. |
| PE | 72.193 | 17.171 | 85.393 | 16.767 | 60.249 | 24.064 | n.s. | n.s. | n.s. |
| PE-P | 1.313 | 0.243 | 0.526 | 0.136 | 0.916 | 0.166 | <0.0001 | 0.0064 | 0.0078 |
| PG | 6.198 | 1.495 | 12.973 | 4.579 | 3.582 | 1.639 | 0.0061 | 0.0003 | n.s. |
| PI | 41.717 | 7.223 | 39.157 | 8.012 | 44.678 | 9.599 | n.s. | n.s. | n.s. |
| PS | 11.296 | 1.444 | 1.200 | 0.335 | 8.494 | 1.630 | <0.0001 | <0.0001 | 0.0066 |
| SM | 8.185 | 0.619 | 3.090 | 0.662 | 5.834 | 1.642 | <0.0001 | 0.0020 | 0.0092 |
| TAG | 17.082 | 1.565 | 7.313 | 0.964 | 53.924 | 11.260 | n.s. | <0.0001 | <0.0001 |
DC, differential centrifugation; UC, ultracentrifugation; MACS, magnetic bead-assisted isolation; CE, cholesteryl ester; CER, ceramide; CL, cardiolipin; DAG, diacylglycerol; FFA, free fatty acids; HexCER, hexosyl-ceramide; LPC, lyso-phosphatidylcholine; LPE, lyso-phosphatidylethanolamine; LPI, lyso-phosphatidylinositol; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PE-P, phosphatidylethanolamine plasmalogen; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserine; SM, sphingomyelin; TAG, triacylglycerol. n.s., not significant. Values are means of at least 5 purifications.