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. 2016 Feb 19;6:21266. doi: 10.1038/srep21266

Figure 1. Design and assembly of AM-DNA origami.

Figure 1

(a) The crystal structure of PfLDH with two aptamers bound (pdb 3ZH210). PfLDH is shown in cartoon format with each monomer coloured a different colour. Aptamer strands are shown in stick format and coloured red. (b) DNA origami is made by mixing the M13 DNA strand with staple strands. 12 staple strands were modified with additional sequences (red) corresponding to a linker sequence followed by the aptamer sequence. (c) After annealing, the rectangular DNA origami was formed with the 12 aptamer sequences protruding from the surface (red). (d) PfLDH (grey circle) is mixed with the modified DNA origami. (e) Immobilisation of PfLDH on the rectangular DNA origami surface. (f) Schematic of DNA origami strand arrangement. The M13 template is shown in black and the staple strands are shown in red. Red staple strands on the right of the structure are those which were modified by addition of the aptamer sequences while strands 127, 129, 150, 151, 152, 153 are the index staples. Staple strands are named as in Table 1.

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