Figure 3. G₁ lengthening in activated NSCs at 6 months.

FUCCI mice (red and green fluorescent) were used to evidence an increase of the G₁ proportion (FUCCI-Red cells) associated with a decrease of the S-G₂/M proportion (FUCCI-green cells) specifically in (A) LeX⁺ EGFR⁺ cells, but not in (B) EGFR⁺ cells in 6-month-old mice. Videomicroscopy of (C) LeX⁺ EGFR⁺ and (D) EGFR⁺ cells sorted from FUCCI-Red mice allowing the tracking of the G₁ phase with red fluorescence and the S-G₂/M phase (colourless). (E) LeX⁺ EGFR⁺ and EGFR⁺ cells presented a similar S-G₂/M phase length at 6 months, whereas (F) LeX⁺ EGFR⁺ cells had a longer G₁ phase. The data are presented as the mean ± SEM and were obtained from analyses of cells isolated from at least 3 independent mice (A,B) and isolated cells from 2 independent groups of 3 to 5 mice (C–F) for each time point. *p < 0.05, ***p < 0.001. Scale bar for microscopy, 10 μm.