Figure 4. Immunohistochemistry analysis of the Eker rat (Tsc2+/−) renal tumors shows hyperactivation of ERK1/2 and mTOR kinases within lesions, relative to the surrounding tissue.
Sections were prepared and stained using p-ERK1/2 Thr202/Thr204 (A–C red) and p-mTOR Ser2448 (D–F red) antibodies. Representative sections are shown.