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. 2016 Feb 19;6:21807. doi: 10.1038/srep21807

Figure 5. Immunoblot analysis of kidney lysates.

Figure 5

(A) Blot membranes were incubated with the antibodies against p-ERK1/2 Thr 202/204 and p-mTOR Ser 2448. The mean integrated optical density is related to actin for p-ERK1/2 (B) and p-mTOR (C) activity. Results are given as a mean ± S.E.M. KD4, KD6, KD8 – groups of Eker rats (Tsc2+/−) treated with HFKD for four, six or eight mo., respectively; ST – Eker rats fed with a standard diet; LE ST – wild-type Long Evans rats treated with a standard diet; LE KD – wild-type Long Evans rats treated with a ketogenic diet similarly to the KD6 group. *P < 0.05 as compared to ST for ANOVA with a Fisher post hoc test.