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. 2016 Feb 19;36(1):e00299. doi: 10.1042/BSR20150267

Figure 6. HKII expression knockdown did not lead to changes in AKT phosphorylation following 3BP exposure.

Figure 6

HKII expression in HCT116 and DLD-1 cells were knocked down using RNA interference. Both siRNA sequences used showed efficient knockdown of HKII expression following 24 h transfection (A). Representative blots from DLD-1 cells are shown. Recovery time of CRC cells following siRNA transfection (B). Expression of AKT and related proteins following 4 h 3BP dose–response on HCT116 (C) and DLD-1 cells (D). siRNA experiments were performed twice with equivalent results.