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. 2016 Jan;136(1):99–106. doi: 10.1038/jid.2015.383

Figure 2.

Figure 2

Epidermal penetration by bacteria. (a and b) Whole-mount FISH of ear skin using BacUni (a) or C. dubuliniensis (b) probes (green), with DAPI counterstain (blue). The images are Z-stack maximum projections of the epidermis. Scale bars = 20 μm. (c) Optical sectioning of 3D reconstructed whole-mount FISH (BacUni). Arrowheads show bacterial penetration of the basal epidermal layer. Dotted line indicates position of basement membrane. Scale bars = 10 μm. (d) Quantitation of location of individual BacUni signals relative to the median thickness (dotted line) of the epidermis. The boxes extend from the 25th to 75th percentiles. Whiskers indicate minimum to maximum. ****P < 0.0001.