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. 2015 Oct 23;36(2):426–441. doi: 10.1177/0271678X15609939

Figure 3.

Figure 3.

Treatments with small molecules identified by qHTS increase the levels of SUMO conjugation and the Ubc9 conjugase in SHSY5Y parent cells. (a) Representative immunoblots of high molecular weight (>100 kDa) SUMO-1 and SUMO-2,3 conjugates and the Ubc9 protein in the total cell lysates from SHSY5Y cells treated with various compounds with indicated concentrations for 13.5 h. (b) Quantitative analyses of the conjugates and Ubc9 from three independent experiments. High molecular weight SUMO-1 or SUMO-2,3 conjugates (>100 kDa) were cropped in each lane and the total intensity measured. The densities were normalized to corresponding actin levels and expressed as the ratio to control (DMSO alone). Data represent the mean+/−standard deviation of three independent experiments. **p < 0.01, *p < 0.05 compared to DMSO control.