The effect of small molecules identified by
qHTS on OGD/ROG-induced cell death in SHSY5Y cells and E18 rat
cortical neurons. After OGD/ROG exposure (OGD/ROG:
16 h/5 h for SHSY5Y, 5 h/16 h for
cortical neurons) in the presence or absence of the small molecules
indicated, cell death/survival was assessed via nuclear staining
with Hoechst 33342 and propidium iodide (PI) followed by FACS
analyses. The percentage of total cell death (both apoptotic and
necrotic) was calculated and recorded vs the percentage of
0.1% DMSO control cell death in each experiment. (a) SHSY5Y
cells. (b) Rat cortical neurons. (c) Increasing concentrations of
AHPN displayed statistically significant increases in viability and
decreases in cell death after OGD/ROG as measured by FACS analysis.
Upper panels: representative dot plots without AHPN (left) and with
1 µM AHPN (right) with numbers in each population
(left, apoptotic cells; upper right, necrotic cells; lower right,
viable cells); lower panels: Quantitative analyses of cell survival
(left) and cell death (right). (d) Increasing concentrations of AHPN
displayed statistically significant decreases in cell death as
measured by LDH release. Data represent the
means ± standard deviation of three
independent experiments.
**p < 0.01,
*p < 0.05
compared with OGD/ROG without AHPN by student's
t-test.