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. 2016 Feb 19;6:21145. doi: 10.1038/srep21145

Figure 1. Effect of ICA treatment on the viability and morphology of human ESCC cells.

Figure 1

(A) EC109 cells were treated with increasing concentrations of ICA (20, 40, or 80 μM) and assessed at different time points (12, 24, and 36 h). The cell viability is expressed as OD values. (B) TE1 cells were treated with increasing concentrations of ICA (20, 40, or 80 μM) and assessed at different time points (12, 24, and 36 h). The cell viability is expressed as OD values. The morphology of both of the ESCC lines was observed under an inverted phase-contrast microscope after the cells were treated for 24 h, and images were obtained. Significant cell shrinkage and a decreased cellular attachment rate were observed in the ICA treatment groups. All of the results are expressed as the mean ± SD; n = 6. aP < 0.05 vs. the control group; bP < 0.05 vs. the 20 μM ICA-treated group; cP < 0.05 vs. the 40 μM ICA-treated group.