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. 2016 Feb 19;6:21145. doi: 10.1038/srep21145

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(A) Viability is shown as OD values. (B) The intracellular Caspase 9 activity levels are shown. (C) ROS concentrations are shown. (D) NADPH oxidase activity is shown. The two indexes in the control group were defined as 100%. (E) Representative Western blot results of p-eIF2α, CHOP, Bcl2, and PUMA are shown. Membranes were re-probed for β-actin expression to show that similar amounts of protein were loaded in each lane. The results are expressed as the mean ± SD; n = 6. ^aP < 0.05 vs. the control siRNA group; ^bP < 0.05 vs. the eIF2α siRNA-treated group; ^cP < 0.05,vs. the control siRNA + ICA 40 μM-treated group.