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. 2016 Feb 19;6:21420. doi: 10.1038/srep21420

Figure 4. Conditioned media from MSCs contains cytokines and induces expression of pluripotency factors in CRC cell lines.

Figure 4

(A) ELISA analysis of the levels of IL-6 and IL-8 in HCT-116 conditioned media, MSC-CM, concentrated MSC-CM (50×) and control media. (B) HCT116 cells were cultured in MSC-CM or control media for 24 h and the mRNA expression levels of C-myc, Sox2 and OCT4 were analyzed using Q-PCR. (C) Western blot analysis of c-Myc and Sox-2 protein expression. (D) Quantification of c-Myc and Sox-2 expression from Western blots. *P  < 0.05 and **P < 0.01 vs. control group (n > 3).