Table 3.

Prevalence of pfhrp2− in false negative PfHRP-2 RDT test determined by PCR

	PfHRP-2 RDT−	PfHRP-2 RDT+
pfhrp2 PCR− (Accra)	23 % (6/26)	36 % (34/94)
pfhrp2 PCR+ (Accra)	77 % (20/26)	64 % (60/94)
pfhrp2 PCR− (Cape Coast)	0 % (0/12)	14 % (1/7)
pfhrp2 PCR+ (Cape Coast)	100 % (12/12)	86 % (6/7)

Samples that were confirmed as P. falciparum positive by PCR genotyping were grouped according to their PfHRP-2 RDT result, PfHRP-2 RDT positive (PfHRP-2 RDT+) or PfHRP-2 RDT negative (PfHRP-2 RDT−). pfhrp2 exon 2 PCR amplification was performed on each sample to estimate effect of the presence of absence of pfhrp2 on malaria diagnosis by PfHRP-2 RDT kits