Fig. 1.

Skeletal development, body weight and skeletal muscle development. A comparison of alizarin blue staining of wild type (a) and FKRP^KD (b) pups at P0 indicated no differences with respect to skeletal development. c Body weight was significantly lower in FKRP^KD (p = 0.0069) relative to wild type; there was no significant difference in body weight between wild type and heterozygous mice. Tibia/femur length was measured using micro-computed tomography and showed no significant differences between FKRP^KD and wild type at P0. Data was analysed by one-way ANOVA. d Transverse sections of the hindlimb muscle at E15.5 and P0 immunolabelled for slow myosin heavy chain. The proportion of myotubes expressing slow myosin heavy chain was similar between FKRP^KD and wild type. At P0, fewer myotubes/fibres expressed slow myosin, with those still positive distributed in the superficial area of the TA and EDL. In the soleus which is a slow postural muscle in the adult, slow myosin continued to be expressed in most of myotubes/fibres