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. 2016 Feb 19;6:3. doi: 10.1186/s13395-016-0073-y

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© Kim et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Myoblast fusion at E15.5. Myoblasts were isolated from the limbs at E15.5 for the further evaluation of reduction in cluster size that we observed on transverse sections. The myogenic fusion index calculated by counting the proportion of nuclei contained within myotubes showed a significant reduction in FKRP^KD relative to wild type (a). Desmin immunolabelling further showed that the number of myotubes that formed 4–5 days after plating on Matrigel pre-coated coverslips was reduced in the FKRP^KD (n = 6 wild type and 6 FKRP^KD pups) (b)