Table 1.
Antibodies used in immunohistochemistry
| Antibodies | Manufacturer | Dilution | Species |
|---|---|---|---|
| α-dystroglycan (IIH6) | Millipore | 1:200 | Mouse (IgM) |
| β-dystroglycan | Vector laboratories | 1:20 | Mouse |
| Pax7 | Developmental Studies Hybridoma Bank (DSHB) | 1:50 | Mouse |
| Ki67 | DAKO | 1:200 | Rat |
| Type IV collagen | Invitrogen | 1:50 | Goat |
| Perlecan | Invitrogen | 1:5000 | Rat |
| Pan Laminin | Sigma | 1:200 | Rabbit |
| Laminin α1 | Gift from Professor Madeleine Durbeej-Hjalt | 1:100 | Rat |
| Laminin α2 | Enzo | 1:200 | Rat |
| Laminin α4 | R&D systems | 1:200 | Goat |
| Laminin α5 | Gift from Madeleine Durbeej-Hjalt | 1:200 | Rabbit |
| Laminin β1 | Millipore | 1:2000 | Rat |
| Laminin γ1 | Millipore | 1:2000 | Rat |
| Fast myosin heavy chain | Leica | 1:20 | Mouse |
| Slow MHC | Millipore | 1:200 | Mouse |
| Developmental MHC | Leica | 1:20 | Mouse |
| Mandys1 (dystrophin) | Gift from Professor Glenn Morris | 1:20 | Mouse |
| Myogenin | Santa Cruz | 1:200 | Rabbit |
| MyoD | Santa Cruz | 1:200 | Rabbit |