Skip to main content
. Author manuscript; available in PMC: 2016 Feb 19.
Published in final edited form as: Endocrinology. 2014 Feb 7;155(4):1222–1234. doi: 10.1210/en.2013-1646

Figure 3.

Figure 3

Effect of PBF on p53 ubiquitination. A, Detection of high mwt p53 conjugates by Western blot analysis in TPC1 cells transfected with either VO or PBF and then treated with 10 μM MG132. B-C, Western blot analysis of p53 in TPC1 cells transfected with either VO or PBF and then incubated with 50 μM nutlin-3 prior to 100 μM anisomycin treatment. DMSO was used as vehicle. D, Mean p53 protein levels relative to β-actin quantified from 3 independent experiments are shown. Data presented as mean ± SE. *, P < .05; NS = not significant.