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. 2016 Feb 16;36(5):756–764. doi: 10.1128/MCB.00930-15

FIG 6.

FIG 6

ActivinB decreases the expression of mature β-cell genes while promoting Pax4 expression. (A and B) Quantitative RT-PCR analysis of mature β-cell gene expression in Min6 (A) or βTC3 (B) cells following 72 h of activinB stimulation. (C) Analysis of MafA, Nkx6.1, Nkx,2.2 and Pdx1 protein expression in Min6 cells subjected to 72 h of activinB stimulation (+). Tubulin was used as a loading control. (D and E) Analysis of Pax4 gene (D) and protein (E) expression in βTC3 cells subjected to 72 h of activinB (ActB) stimulation in the presence or absence of SB431542 (SB). Graphs are representative examples of 3 independent experiments. Data are presented as mean ± SEM for triplicate samples per experiment. *, P < 0.05; **, P < 0.01; ***, P < 0.001; NS, not significant (by the Student t test).