FIG 10.

hGRβ gene regulation profile in GRLKO mice. Two-month-old GRLKO mice were injected with PBS (n = 8), AAV-GFP (n = 8), and AAV-hGRβ (n = 8). (A) Western blotting of the indicated liver samples was performed 1 month after injection with anti-GR antibody (D8H2), which recognizes both GRα and GRβ. Liver samples collected from each treatment group were compared side by side. (B) Serum ALT, ALP, ALB, and Tpro were tested for different groups of GRLKO mice. No significant difference was found among the groups for each test. (C) Total RNA isolated from each group of GRLKO mice 1 month after injection was applied to an Agilent whole-mouse one-color array. Shown are Venn diagrams of AAV-GFP-regulated genes (left) and AAV-hGRβ-regulated genes (right). The common part (330 genes) indicates AAV backbone effect. The unique part (1,670 genes) of AAV-hGRβ represents hGRβ-regulated genes in GRLKO mice, about 60% of which are upregulated. (D) Ingenuity pathway analysis predicted the top biological functions of hGRβ-regulated genes in GRLKO. (E) Blood glucose profiles of PTTs in GRLKO mice. Two months after AAV injection, blood glucose levels were determined after 18 h of fasting. hGRβ expression did not significantly affect hepatic gluconeogenesis compared to other GRLKO controls (n = 6). (F) RT-PCR analysis of PEPCK from GRLKO livers. hGRβ expression does not significantly change PEPCK gene expression in GRLKO livers compared to other GRLKO controls (n = 6; P < 0.05). The error bars indicate standard errors of the means.