FIG 6.

(A) Upregulation of type I and II IFN, STAT1, and SOCS1 gene expression in wild-type mouse liver expressing hGRβ. The total liver RNA extracted from each group was applied to an Agilent whole-mouse one-color array and used for RT-PCR. The interferon signaling pathway was changed after AAV-hGRβ injection. Gene sets in hGRβ-injected liver were overlaid on the interferon signaling pathway. Interferon, STAT1, SOCS1, and some interferon-stimulated genes were upregulated. Red represents upregulated genes. (B) RT-PCR confirmed the upregulation of genes shown in panel A. Relative RNA values were normalized to PPIB. STAT1, SOCS1, IFN-β1, and IFN-γ and were significantly increased in the AAV-hGRβ-treated group compared to the control group. No significant change was found in IFN-γRα, IFN-γRβ, and IFN-αR2 (*, P < 0.05). The error bars indicate standard errors of the means.