FIG 3.

Effects of Vps1 mutations on behavior of an early endocytic marker, Sla1-GFP. (A) The lifetimes of Sla1-GFP at endocytic sites were measured. Mean lifetimes and numbers of patches counted are shown. Only the vps1 deletion strain showed a lifetime significantly different from that of the wild-type strains. (B) The behavior of Sla1-GFP patches was assessed and categorized as indicated. Patches were considered to endocytose normally, show no invagination, show retraction, or show invagination followed by a delay in the invaginated state. Examples of kymographs depicting these behaviors are shown. Error bars indicate standard deviations.