FIG 6.

Effect of S599 mutation on ultrastructure of plasma membrane invaginations. (A) Cells expressing the wild type or vps1 mutations were high-pressure frozen and processed for electron microscopy as described in the text. The lengths of invaginations observed in cells were measured. Each spot represents one measurement, and means are indicated. Both S599D and S599V cause a statistically significant increase in invagination length. Error bars indicate standard deviations. (B) Images of wild-type invaginations, as well as average and long invaginations for the S599V and S599D mutants.