TABLE 2.
Plasmids used in this study
| Plasmid | Description | Origin or reference |
|---|---|---|
| pKA552 | Gal4BD-Vps1(276-704) TRP1 | E. Hettema laboratory |
| pKA167 | pGBD-C3::Gal4BD TRP1 | 40 |
| pKA163 | pGAD-C2::GalAD LEU2 | 40 |
| pKA730 | GalAD-Rvs167 LEU2 | 66 |
| pKA777 | Gal4BD-Vps1(276-704) S599V TRP1 | This study |
| pKA778 | Gal4BD-Vps1(276-704) S599D TRP1 | This study |
| pKA544 | URA3; CEN with PGKterm | |
| pKA677 | pKA544 with VPS1 (inc 320bp 5′) | This study |
| pKA781 | pKA544 with vps1 S599V | This study |
| pKA782 | pKA544 with vps1 S599D | This study |
| pKA836 | YCplac111-VPS1-GFP own promoter; LEU CEN | 14 |
| pKA859 | YepLac112-RVS167 TRP1 2μ | Hicke Laboratory |
| pKA902 | pKA836 with vps1 S599V | This study |
| pKA903 | pKA836 with vps1 S599D | This study |
| pKA695 | YCplac111-pTPI1 3×HA-VPS1 LEU CEN | This study |
| pKA904 | pKA695 with vps1 S599V | This study |
| pKA905 | pKA695 with vps1 S599D | This study |
| pKA850 | His-tagged Vps1 wild type | 14 |
| pKA971 | pKA850 with vps1 S599V | This study |
| pKA972 | pKA850 with vps1 S599D | This study |
| pKA1051 | pGal1/10-GST-GST-PHO85 | Open BioSystems |