Table II. Myeloid cell percentages in BM, spleen, and blood.
| 3 d | 7 d | 12 d | 16 d | |
|---|---|---|---|---|
| % Monocytes in total BMCs | ||||
| Naive | 10.14 ± 0.51 | 10.14 ± 0.51 | 10.14 ± 0.51 | 10.14 ± 0.51 |
| WT-EAE-PBS | 15.10 ± 0.96 | 16.39 ± 1.19 | 12.84 ± 1.15 | 12.88 ± 0.39 |
| WT-EAE-Nic | 14.05 ± 0.72 | 16.00 ± 1.69 | 14.13 ± 1.34 | 12.01 ± 0.55 |
| % Monocytes in total spleen cells | ||||
| Naive | 2.03 ± 0.18 | 2.03 ± 0.18 | 2.03 ± 0.18 | 2.03 ± 0.18 |
| WT-EAE-PBS | 2.45 ± 0.21 | 2.91 ± 0.24 | 2.87 ± 0.30 | 2.48 ± 0.46 |
| WT-EAE-Nic | 3.02 ± 0.45 | 3.11 ± 0.43 | 4.50 ± 0.48 | 2.44 ± 0.39 |
| % Monocytes in total blood cells | ||||
| Naive | 8.16 ± 0.74 | 8.16 ± 0.74 | 8.16 ± 0.74 | 8.16 ± 0.74 |
| WT-EAE-PBS | 5.41 ± 1.61 | 8.49 ± 1.82 | 11.28 ± 2.10 | 13.48 ± 1.85 |
| WT-EAE-Nic | 4.52 ± 1.04 | 8.28 ± 5.91 | 9.23 ± 5.31 | 7.65 ± 1.69 |
| α7KO-EAE-PBS | — | — | — | 18.39 ± 5.07 |
| α7KO-EAE-Nic | — | — | — | 13.74 ± 3.77 |
| α9KO-EAE-PBS | — | — | — | 12.62 ± 2.54 |
| α9KO-EAE-Nic | — | — | — | 13.06 ± 3.35 |
| % CCR2+Ly6Chigh in total BMCs | ||||
| Naive | 4.75 ± 0.31 | 4.75 ± 0.31 | 4.75 ± 0.31 | 4.75 ± 0.31 |
| WT-EAE-PBS | 6.19 ± 0.78 | 8.60 ± 1.47 | 6.94 ± 0.70 | 5.30 ± 0.10 |
| WT-EAE-Nic | 6.65 ± 0.53 | 8.65 ± 1.42 | 7.11 ± 0.63 | 6.24 ± 0.29 |
| % CCR2+Ly6Chigh in total spleen cells | ||||
| Naive | 0.19 ± 0.04 | 0.19 ± 0.04 | 0.19 ± 0.04 | 0.19 ± 0.04 |
| WT-EAE-PBS | 0.54 ± 0.07 | 0.81 ± 0.15 | 0.75 ± 0.11 | 0.61 ± 0.19 |
| WT-EAE-Nic | 0.59 ± 0.14 | 0.96 ± 0.21 | 1.17 ± 0.22 | 0.47 ± 0.10 |
| % CCR2+Ly6Chigh in total blood cells | ||||
| Naive | 2.20 ± 0.50 | 2.20 ± 0.50 | 2.20 ± 0.50 | 2.20 ± 0.50 |
| WT-EAE-PBS | 1.20 ± 0.46 | 2.04 ± 0.65 | 2.80 ± 1.20 | 3.68 ± 0.67 |
| WT-EAE-Nic | 1.27 ± 0.52 | 2.33 ± 1.19 | 2.58 ± 0.90 | 1.83 ± 0.43 |
| α7KO-EAE-PBS | — | — | — | 3.64 ± 0.89 |
| α7KO-EAE-Nic | — | — | — | 3.56 ± 0.93 |
| α9KO-EAE-PBS | — | — | — | 3.04 ± 1.00 |
| α9KO-EAE-Nic | — | — | — | 2.44 ± 0.44 |
| % Neutrophils in total BMCs | ||||
| Naive | 34.12 ± 2.87 | 34.12 ± 2.87 | 34.12 ± 2.87 | 34.12 ± 2.87 |
| WT-EAE-PBS | 41.55 ± 2.83 | 52.83 ± 2.40 | 58.40 ± 2.43 | 58.62 ± 2.38 |
| WT-EAE-Nic | 47.96 ± 2.99 | 53.12 ± 1.69 | 60.02 ± 2.19 | 63.21 ± 2.42 |
| % Neutrophils in total spleen cells | ||||
| Naive | 0.76 ± 0.10 | 0.76 ± 0.10 | 0.76 ± 0.10 | 0.76 ± 0.10 |
| WT-EAE-PBS | 5.76 ± 1.46 | 6.84 ± 1.58 | 7.31 ± 1.06 | 4.48 ± 0.77 |
| WT-EAE-Nic | 4.45 ± 1.52 | 5.73 ± 0.88 | 9.33 ± 1.10 | 6.80 ± 1.28 |
| % Neutrophils in total blood cells | ||||
| Naive | 19.04 ± 2.92 | 19.04 ± 2.92 | 19.04 ± 2.92 | 19.04 ± 2.92 |
| WT-EAE-PBS | 36.74 ± 9.79 | 57.87 ± 6.30 | 76.10 ± 4.04 | 46.70 ± 10.12 |
| WT-EAE-Nic | 24.51 ± 8.45 | 47.60 ± 5.03 | 72.46 ± 7.48 | 68.45 ± 9.01 |
The average percentages of each subpopulation of myeloid cells with respect to the total number of cells in the BM, spleen, or blood in each group and time point are indicated ± SEM. Dashes indicate that no data were collected at these time points.