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. 2016 Feb 1;129(3):592–603. doi: 10.1242/jcs.178285

Fig. 5.

Fig. 5.

MyRIP–actin interaction regulates WPB trafficking. (A) Representative x-y trajectories of individual WPBs in single HUVECs expressing VWFpp–EGFP (i), EGFP–MyRIP WT (ii), EGFP–MyRIP A751P (iii), EGFP–MyRIP 4A (iv) or EGFP–MyRIP A751P 4A (v). Trajectories were determined here and elsewhere from TIRFM videos using the ASPT function of GMimPro software (see Materials and Methods). The number of cells imaged and trajectories detected were: VWFpp, n=15 cells, 3890 trajectories; MyRIP WT, n=11 cells, 2978 trajectories; MyRIP A751P, n=13 cells, 1278 trajectories; MyRIP 4A, n=8 cells, 1862 trajectories; MyRIP A751P 4A, n=11 cells, 1310 trajectories. (B,C) Mean±s.e.m. parameters determined from detected trajectories of long-range (Bi–iii) or short-range (C) movements of WPBs in HUVECs expressing EGFP fusion proteins of VWFpp, MyRIP WT and MyRIP mutants as indicated. Number of WPBs analysed for short-range movements were: VWFpp, n=277; MyRIP WT, n=86; MyRIP A751P, n=242; MyRIP 4A, n=250; MyRIP A751P 4A, n=387. P values are with respect to VWFpp–EGFP (one-way ANOVA using Tukey multiple comparisons test).